‘Two-photon (2-P) microscopy offers several advantages for biological imaging – in particular for non-injurious imaging of dynamic cell behaviors deep within intact tissues, organs and even the living animal [Cahalan et. al., 2003, Stutzmann et al., 2005]. However, its widespread adoption for such applications has been hindered by two factors: commercial 2-P microscopes are very expensive, and they typically acquire images at frame rates too slow to resolve many biological processes. Both of these problems may be circumvented by building your own 2-P microscope!
[..] The object of this web page is to gather together all the information you should need to build your own 2-P microscope.’